This Glutamate Synthase Assay Kit is a quantitative assay kit for measuring Glutamate Synthase activity in animal and plant tissue, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Glutamate synthase consumes NADH during transfer of an amino group from glutamine to alpha-ketoglutarate. Activity is measured by the decrease in absorbance at 340 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal and plant tissues, cells, bacteria, serum, and plasma
Assay Time
30-60 minutes
Components
Reagent I, Reagent II, Reagent III
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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