The GSSG/GSH Quantification kit contains Masking Reagent of GSH. The GSH can be deactivated in the sample by simply adding the Masking Reagent. Therefore, only the GSSG is detected by measuring the absorption (?max = 412 nm) of DTNB (5,5’-dithiobis (2-nitrobenzoic acid) using the enzymatic recycling system. Also, GSH can be determined the quantity by subtracting GSSG from the total amount of glutathione. The kit is limited to quantifying GSH/GSSG concentration from 0.5 µmol/l to 50 µmol/l and GSSG concentration from 0.5 µmol/l to 25 µmol/l.
Applications
SOD Activity, Glutathione, DNA Damage, Antioxidant capacity Kits for Life Science Research
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Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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