Human Brain Organoid Development Kit (A334706)

Human Brain Organoid Development Kit

This kit enables the generation and maintenance of human or animal brain organoids in vitro, providing optimal conditions for neural tissue development.

Cell culture, 3D Cell Culture, Organoids

The organoid culture assay is based on the ability of primary tissue-derived cells to self-organize into three-dimensional structures when provided with an appropriate extracellular matrix and a defined, lineage-specific growth environment. Fresh tissue is first processed using a controlled enzymatic digestion solution that releases viable stem and progenitor cell clusters while preserving surface receptors and cellular polarity. The resulting fragments are embedded in a matrix such as Matrigel, which functions as a scaffold that supports 3D architecture and enables signaling through integrins and basement-membrane interactions. The cultures are then supplied with a specialized organoid medium containing niche-mimicking growth factors, small molecules, and nutrients that maintain stemness while permitting tissue-specific differentiation. During routine expansion, a gentle passaging enzyme is used to fragment mature organoids into smaller units without compromising viability, allowing serial propagation over many passages. For biobanking, a cryopreservation formulation containing permeable and non-permeable cryoprotectants is applied to prevent ice-induced damage and maintain long-term genomic and functional stability. After thawing, a recovery buffer restores isotonic balance and supports rapid re-establishment of 3D growth. Together, this workflow enables the continuous generation, expansion, freezing, and re-culturing of physiologically relevant organoids that retain genetic, phenotypic, and microenvironmental features of the source tissue, making the system suitable for disease modeling, drug screening, and personalized research applications.

The kit contains Brain organoid medium A in a 100 mL volume, which is used to support the growth and maintenance of brain organoids and can be stored at +4 °C for up to three months or at -20 °C for long-term storage of up to one year. It also includes Primary Buffer B2 (250 mL), which is formulated to prepare and stabilize samples during early culture steps and remains stable at 4-20 °C for two years. Primary tissue digestive juice C (50 mL) is provided for enzymatic dissociation of brain tissue and can be stored at 4-20 °C for one year. The set also supplies Organoid passage digestive juice D (50 mL), used to break down mature organoids for subculture, with the same storage requirements. Tissue preservation solution E (100 mL) is included to temporarily store tissue prior to processing, and Organoid Cryopreservation Solution F (20 mL) is supplied for freezing and long-term storage of established organoids, both stable for one year and two years respectively at 4-20 °C. Finally, Passage Buffer G2 (250 mL) is used to wash and resuspend cells during passaging and can be stored at 4-20 °C for two years.

Liquid

Shipped at 4°C. Store at 4°C with a shelf life of 3 months; store at -20°C with shelf life of 1 year.

Proper storage is essential to maintain reagent performance. Brain organoid medium A should be kept at 4 °C and used within 1 month, or stored at -20 °C for long-term use, avoiding more than two freeze-thaw cycles. Tissue preservation solution E and digestion solution C must also be stored at -20 °C under the same conditions to retain nutrient activity. Refer to the Product Manual for full instructions.