When excited at 680nm, Anti-Human IgG Fc Fluorescent Beads 680/615 generate a bright chemiluminescence detectable at 615nm. Since they are embedded with a very bright Eu3+ chelate, these beads can emit intense fluorescence when excited around 340nm (detectable using Fluorescence Intensity or Time Resolved Fluorescence modes). Anti-Human IgG Fc Fluorescent Beads 680/615 are functionalized with antibodies specific to the Fc moiety of human IgGs. These beads allow efficient capture of human IgGs through their heavy chains so their Fab's are optimally exposed to interact with antigens of interest. These beads can be used to directly replace HRP labeled anti-human IgG's during straightforward ELISA conversion to FLISAs (Fluorescent Linked Immunosorbent Assay).
Applications
Protein-Protein Interactions (PPi's) Assays
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in storage buffer at a concentration of 5 mg/ml.
Storage
Shipped at 4°C. Store undiluted in the dark at 4°C.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Anti-Human IgG Fc Fluorescent Beads 680/615 (A334451)
Direct detection of Human IgG with Anti-Human IgG Fc Fluorescent Beads 680/615. Anti-Human IgG Fc Fluorescent Beads (10 µg/ml) were added to opaque wells pre-coated with 0-1000 ng Human IgG and excited at either 340 nm (blue line) or 680 nm (red line), with detection at 615 nm. EC50 = 151 ng/well.
Anti-Human IgG Fc Fluorescent Beads 680/615 (A334451)
Fluorescent Beads enhance ELISA sensitivity and can replace traditional chromogenic methods. In a sandwich ELISA, streptavidin-coated fluorescent beads are added to bind to the biotinylated detection antibody. After removal of unbound beads, bound fluorescent beads are excited at either 680nm or 340nm to result in 615nm emission. No stop reaction is needed.
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