This Hydrogen Peroxide Assay Kit is a quantitative assay kit for measuring Hydrogen Peroxide in serum, plasma, urine, animal and plant tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Hydrogen peroxide oxidizes ferrous ions to ferric ions, which then react with xylenol orange to form a purple complex. The signal measured at 580 nm is proportional to hydrogen peroxide concentration.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, urine, animal tissues, plant tissues, cells, and bacteria
Assay Time
20-40 minutes
Components
Reaction Buffer, Hydrogen Peroxide Standard (1M), Assay Buffer (10X)
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well clear flat bottom microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
