Distinguishing between live and dead cells is very important for investigation of growth control and cell death. This kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells.
Platform
Flow Cytometer, Fluorescence Microscope
Sample Type
Cells
Components
LiveDye, NucleiDye, Assay Buffer (10X)
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
The red dye is a potential mutagen. Take appropriate precautions when handling this reagent.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunocytochemistry - Live and Dead Cell Double Staining Kit (A319650)
Hela cells stained with Live and Dead Cell Double Staining Kit (A319650). A: Live cells stain green, B: Dead cells (30 minute incubation in 70% ethanol) stain red.
