Description
This kit is available in 5 µg and 20 µg sizes, the measurements of their column binding capacities, meaning they can extract up to 5 µg or 20 µg of DNA respectively. The LumiPure DNA Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from agarose gels or enzymatic reactions. The DNA fragment of interest is excised from an agarose gel and placed in a microcentrifuge tube. Gel Solubilization Buffer dissolves the agarose gel slice, denatures proteins and ensures selective binding of DNA to the membrane in the column. It also contains a color pH indicator that enables easy control of the optimal pH. If pH shifts toward the alkaline side, then adding of Neutralization Buffer returns the optimal pH value for DNA binding to the column membrane. In the following step, a series of washings of the column membrane containing DNA are performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the DNA is eluted with Elution Buffer or, if necessary, with deionized water. Elution volumes for the two kit sizes are 10 µl and 25 µl. Typical DNA recovery is 85-95%. DNA purity, OD260/OD280, is greater than 1.8. The purified DNA is suitable for any subsequent application, such as PCR, restriction enzyme digestion, ligation and transformation, labeling, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.
