Description
The LumiPure genomic DNA Blood and Buccal Kit is designed to isolate total (genomic, mitochondrial, and viral) DNA from biological samples using spin columns. The kit includes all the necessary components. First, cell lysis is performed using Lysis Solution BB and Proteinase K. Lysis Solution BB allows selective binding of DNA to the membrane in the column. In the following step, a series of washings of bound DNA are performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the genomic DNA is eluted with Elution Buffer or, if necessary, with deionized water. Column binding capacity of 20 µg DNA. DNA typical yield: whole blood (100 µL) – 1-3 µg; buccal swab – 0.5-2 µg; cultured cells (1 × 106) – 3-6 µg. DNA quality: 30-50 kb in size, OD 260/280 of 1.7-1.8. The isolated DNA is suitable for PCR, restriction enzyme digestion, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.
