Principle of Assay
Macrodantin Strip Test Kit (A327224) uses a competitive colloidal gold based format. The sample is added to the test well to allow the Macrodantin binding protein on the gold particles to engage with any Macrodantin present in the sample. Any gold particles that are not complexed with Macrodantin present in the sample will bind to the Macrodantin imprinted at the Test line (T-line), forming a signal (red line) at that position. If the Macrodantin binding protein on the gold particle has engaged with Macrodantin present in the sample, the gold particle will flow past the T-line and reach the Control line (C-line).
