Components
The kit contains PD-L1 rabbit monoclonal antibody, PD 1 rabbit monoclonal antibody, Ki67 rabbit monoclonal antibody, SOX2 rabbit monoclonal antibody, and GSTP1 rabbit monoclonal antibody, which are used to specifically recognize their respective protein targets in tissue or cell samples. It also includes TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, and TSA monochromatic fluorescent dye 700. These dyes emit green, yellow-orange, red, far-red, and near-infrared light respectively and are used with tyramide signal amplification to visualize multiple targets in the same sample. The kit contains a signal amplification reaction solution, which provides the reagents necessary for the TSA reaction to deposit the fluorescent dye at the site of the target. A Pika universal HRP-labeled secondary antibody is included, which binds to the primary antibodies and catalyzes the TSA reaction. The kit also contains an anti-fluorescence quenching tablet to protect the fluorescent signal from fading during imaging. Finally, DAPI is provided to stain cell nuclei and serve as a counterstain.
