This Mitochondrial Complex IV Activity Assay Kit is a quantitative assay kit for measuring Mitochondrial Complex IV activity in animal tissue , plant tissue and cell samples.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Complex IV oxidizes reduced cytochrome c, causing a decrease in absorbance at 550 nm. This decrease is proportional to enzyme activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, and cultured cells
Assay Time
40-80 minutes
Components
Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V, Reagent VI
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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