This Mitochondrial Isocitrate Dehydrogenase Activity Assay Kit is a quantitative assay kit for measuring Mitochondrial Isocitrate Dehydrogenase activity in animal and plant tissues, and cells (suspension and adherent).
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Isocitrate dehydrogenase reduces NADP+ to NADPH. The resulting increase in absorbance at 340 nm is proportional to enzyme activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, and cultured cells
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well UV microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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