This Mitochondrial Malate Dehydrogenase Activity Assay Kit is a quantitative assay kit for measuring Mitochondrial Malate Dehydrogenase activity in animal and plant tissues, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Mitochondrial malate dehydrogenase reduces oxaloacetate to malate while oxidizing NADH. The decrease in absorbance at 340 nm is used to calculate activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal and plant tissues, cells, bacteria, serum, and plasma
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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