Principle of Assay
This kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This kit is based on enzyme cycling reaction (it does not recognize NADP+/NADPH), in which the formed NADPH reduces a formazan (WST-8) reagent. The intensity of the product color, measured at OD450 nm, is proportional to the NADP+/NADPH concentration in the sample.The following substances interfere and should be avoided in sample preparation: EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
