Nucleostain™ DNA Damage Quantification Kit - AP Site Counting
Description
Colorimetric assay for quantitative detection of abasic (AP) sites in DNA.
Applications
Functional Assays, Functional Studies, SOD Activity, Glutathione, DNA Damage, Antioxidant capacity Kits for Life Science Research
Principle of Assay
AP sites are derivatized with a biotin-tagged probe and subsequently detected via avidin–enzyme conjugates. Enzymatic color development is proportional to the number of abasic sites present in the DNA sample.
Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Nucleostain™ DNA Damage Quantification Kit - AP Site Counting (A57402)
Standard curve for AP site quantification. Absorbance at 650 nm increases linearly with the number of AP sites per 1 × 105 base pairs, enabling quantitative determination of DNA damage.
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