This Phosphoenolpyruvate Carboxykinase / PEPCK Activity Assay Kit is a quantitative assay kit for measuring Phosphoenolpyruvate Carboxykinase / PEPCK activity in animal tissue, plant tissue, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
PEPCK activity is measured in a coupled reaction in which NADH is consumed downstream of oxaloacetate conversion. The decrease in absorbance at 340 nm is proportional to PEPCK activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, bacteria, serum, and plasma
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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