This Phosphoenolpyruvate Carboxylase Activity Assay Kit is a quantitative assay kit for measuring Phosphoenolpyruvate Carboxylase activity in serum, plasma, animal and plant tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
PEPC converts phosphoenolpyruvate and carbon dioxide to oxaloacetate, which is then reduced to malate with NADH consumption. The decrease in absorbance at 340 nm reflects PEPC activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, and bacteria
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III Stock Solution, Reagent III Buffer
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well UV microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Phosphoenolpyruvate Carboxylase Activity Assay Kit (A319716)? Please let us know so that we can list the citation on this page.
