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Phosphoenolpyruvate Carboxylase Activity Assay Kit (A319716)

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$175
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Phosphoenolpyruvate Carboxylase Activity Assay Kit
Description
This Phosphoenolpyruvate Carboxylase Activity Assay Kit is a quantitative assay kit for measuring Phosphoenolpyruvate Carboxylase activity in serum, plasma, animal and plant tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit provides a simple method for detecting phosphoenolpyruvate carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as serum, plasma, animal tissues, plant tissues, cells, and bacteria. In the assay, phosphoenolpyruvate carboxylase (PEPC) catalyzes the reaction of phosphoenolpyruvate and carbon dioxide to produce oxaloacetate and HPO42-. Malate dehydrogenase further catalyzes the reaction of oxaloacetate and NADH to produce malate and NAD+. The rate of NADH decrease at 340 nm reflects phosphoenolpyruvate carboxylase (PEPC) activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, and bacteria.
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III Stock Solution, Reagent III Buffer
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
96-well UV microplate / microquartz cuvette is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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