This kit provides a reliable method for determining protein concentration using the bicinchoninic acid (BCA) assay. The colorimetric reaction allows accurate protein quantification and is suitable for applications such as sample preparation, protein analysis, and biochemical assays.
Applications
WB, Functional Studies
Assay Type
Quantitative
Principle of Assay
This assay is based on reduction of Cu2+ to Cu+ by proteins under alkaline conditions, followed by formation of a colored chelate. The color intensity is proportional to protein concentration over the working range of the assay.
Platform
96-Well Microplate
Sample Type
Protein samples and cell or tissue lysates
Assay Time
30-60 minutes
Components
BCA Reagent A, BCA Reagent B, BSA Standard, BSA Standard Buffer
Storage
Shipped at room temperature. Store at room temperature. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
