Principle of Assay
This kit provides a simple method for detecting pyruvate decarboxylase (PDC) activity in animal and plant tissues, cells, bacteria, serum, and plasma. The principle is that PDC catalyzes the decarboxylation of pyruvate to produce acetaldehyde. Alcohol dehydrogenase then catalyzes the reduction of acetaldehyde by NADH to produce ethanol and NAD+. NADH has an absorption peak at 340 nm, but NAD+ does not. PDC activity is calculated by measuring the rate of decrease in light absorption at 340 nm.
