This Pyruvate Dehydrogenase / PDH Activity Assay Kit is a quantitative assay kit for measuring Pyruvate Dehydrogenase / PDH activity in animal and plant tissues, cells and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Pyruvate dehydrogenase reduces DCPIP during pyruvate oxidation. Enzyme activity is determined by monitoring the absorbance change at 605 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, and bacteria
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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