Description
This kit is intended for the quantification of double-stranded DNA, and is optimized for detection in the broad range with Qubit™ fluorometers (any version can be used). The kit includes QuDye dsDNA BR fluorescent dye concentrate 200X, buffer for the preparation of dye working solution, and DNA concentration standards (0 and 100 ng/µl). This version of the kit also contains thin-walled tubes for use with Qubit™ fluorometers. QuDye dsDNA BR Reagent is selective to double-stranded DNA, so measurement results are not affected by RNA, free nucleotides, or protein contaminants. Other minor contaminants such as salts, detergents, and solvents have a non-significant effect on measurements results. This kit allows accurate dsDNA quantification in the range from 100 pg/µl to 1000 ng/µl for initial samples. The main kit component is QuDye dsDNA BR fluorescent dye, which selectively interacts with DNA and exhibits enhanced fluorescence upon binding to DNA molecules. This kit allows convenient dsDNA quantification in the broad concentration range and does not require a large volume of the initial sample (1–20 µl is required for the assay). All measurements are performed at room temperature and take on average 15–20 minutes (depending on the sample amount). Sample fluorescence is stable for 3 hours. To perform a measurement, prepare dye working solution, dilute DNA standards and experimental samples with it in individual tubes and perform fluorescence measurements using a Qubit™ fluorometer.
