Description
The kit is intended for quantification of single-stranded DNA (ssDNA) with Qubit™ fluorometer. With this kit you can measure concentration of either oligonucleotide or long ssDNA. The kit includes concentrated QuDye ssDNA Reagent, dilution buffer, and ssDNA quantitative standards (0 and 20 ng/uL). This version of the kit also contains thin-walled tubes for use with Qubit™ fluorometers. For quantification of your samples dilute the reagent using the buffer, add samples (sample volume can vary between 1 and 20 uL), and perform fluorescence measurements with a Qubit™ fluorometer. The range of ssDNA concentrations measured is from 50 pg/µL to 200 ng/µL for initial sample (final DNA content in the assay tube after diluting the sample in the dye working solution is 1–200 ng). QuDye ssDNA Reagent also binds to double-stranded DNA (dsDNA) and RNA, so they should be avoided in the ssDNA sample. Free nucleotides and other minor contaminants such as salts, detergents, solvents, and proteins have non-significant effect on measurement results. A wide linear dynamic range of QuDye ssDNA Reagent combined with a small sample volume required for measurements (1–20 uL) makes this kit a quick and convenient tool for quantification of single-stranded DNA.
