With a broad range of excitation wavelengths, R-PE can be easily excited by two laser sources (488 nm & 561 nm), and used in most fluorescence detection applications, e.g. Flow Cytometry (FC), Immunofluorescence microscopy (IF), and Microarrays. Home Algae's R-PE absorbance maxima are at 496, 546, and 565 nm, with a resolvable emission peak at 578 nm. It shows minimal fluoresence quenching.
R-PE won't penetrate into the cell and cause a false-positive reaction.
R-PE has multiple sites for stable conjugation with Biotin, Streptavidin, and other fluorophores, e.g., Cy-3, Cy-5, Cy-7.
Non-toxic, with less chance of affecting characteristics of the sample, human body, and environment.
Sample of Protocol
1. Add the Modifier Solution into the antibody.
2. Incubate the reaction at room temperature for 30 min.
3. Add the Pre-activated R-PE and incubate for 1 hour at room temperature, with agitation and protected from light.
4. Add Quencher into the conjugation mixture and mix completely.
5. Incubate at room temperature and keep away from light for 30 min, until the conjugation is complete.
