This Ribulose Bisphosphate Carboxylase/Oxygenase (RUBISCO) Assay Kit is a quantitative assay kit for measuring RUBISCO activity in plant tissues.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
RUBISCO activity is measured in a coupled reaction leading to NADH oxidation during downstream conversion of reaction products. The decrease in absorbance at 340 nm reflects enzyme activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Plant tissues
Assay Time
40-80 minutes
Components
Extraction Buffer I, Extraction Buffer II, Reagent I, Reagent II, Reagent III, Reagent IV
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
A 96-well UV microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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