Self-immobilizing fluorogenic probe for sensitive detection of ß-galactosidase activity in live cells.
Applications
Fluorescence Microscopy, Confocal Microscopy, High Content Imaging, Flow Cytometry, Intracellular Flow Cytometry, Functional Assays, Senescence and Reporter Gene Assay
Principle of Assay
SPiDER-ßGal is cleaved by ß-galactosidase to generate a reactive quinone methide intermediate that covalently binds to intracellular proteins. This self-immobilizing reaction produces a stable fluorescent signal, enabling sensitive detection at the single-cell level.
CAS Number
1824699-57-1
Molecular Formula
C31H34FNO8
Molecular Weight
567.6
Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Fluorescence imaging of senescence-associated ß-galactosidase activity using SPiDER-ßGal. DAPI (blue) stains nuclei, SPiDER-BGal (orange) marks ß-galactosidase–positive cells, and the merged image shows colocalization. Scale bar: 100 µm.
