This Thioredoxin Reductase Assay Kit is a quantitative assay kit for measuring Thioredoxin Reductase activity in serum, plasma, animal tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Thioredoxin reductase reduces DTNB in an NADPH-dependent reaction to generate TNB. The increase in absorbance at 412 nm is used to calculate enzyme activity after blocking interference from reduced glutathione.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, cells, and bacteria
Assay Time
30-60 minutes
Components
Assay Buffer, Chromogen, TrxR Cofactor, Inhibitor
Storage
Shipped at 4°C. Store at -20°C in the dark for up to 12 months from receipt. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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