This Total Carbohydrate Assay Kit is a quantitative assay kit for measuring Total Carbohydrate in plant tissues, animal tissues, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Total carbohydrates are hydrolyzed to reducing sugars, which reduce DNS under alkaline heating conditions to generate a reddish-brown product. The absorbance at 540 nm is used to determine carbohydrate concentration.
Platform
96-Well Microplate, Microcuvette
Sample Type
Plant tissues, animal tissues, serum, and plasma
Assay Time
60-90 minutes
Components
Hydrochloric acid, Sodium Hydroxide, DNS Reagent, Standard
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
