This Total Cholesterol Assay Kit is a quantitative assay kit for measuring Total Cholesterol in serum, plasma, animal tissues, cells, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Cholesterol esters are hydrolyzed to free cholesterol, which is then oxidized to generate hydrogen peroxide. A peroxidase-coupled reaction forms a red quinone dye measured at 500 nm, proportional to total cholesterol content.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, cells, and bacteria
Assay Time
30-60 minutes
Components
TC Working solution, Standard
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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