Principle of Assay
This kit provides a simple method for determining the triglyceride concentration in a variety of biological samples such as serum, plasma, animal and plant tissues, cells, cell supernatants, and bacteria. In the assay, triglyceride is be extracted by isopropanol and then saponified with KOH to produce glycerol and fatty acids. Then, periodic acid oxidizes glycerol to produce formaldehyde. In the presence of chloride Ions, formaldehyde reacts with acetylacetone to form a yellow substance which has a characteristic absorption peak at 420 nm. The triglyceride present in the sample is proportional to the signal obtained at 420 nm.
