Principle of Assay
Zilpaterol Strip Test Kit (A327265) uses a competitive colloidal gold based format. The extration sample (100 µl) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The extration is incubated briefly (4 min) to allow the Zilpaterol binding proteins on the gold particles to engage with any antibiotic present in the extration sample. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the extration sample will bind to the test line, forming a signal (red line) at that position. Each test strip is imprinted with a Test line (T) and a Control line (C) at the top. If the binding protein on the gold particle has engaged with the Zilpaterol residues present in the extraction sample, the gold particle will flow past the test line and reach the C-line. For visual interpretation of the test results, a test line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the test line which is equal or less intense compared to the C-line indicates the presence of the corresponding Zilpaterol in the extraction sample. The greater the reduction in signal intensity at the test line, the greater the concentration of Zilpaterol residues present in the extraction sample.
