The AEC Coloring System for ELISpot (HRP Substrate) is a chromogenic reagent in which horseradish peroxidase (HRP), in the presence of hydrogen peroxide (H2O2), catalyzes colorless AEC (3-amino-9-ethylcarbazole, C14H14N2) to produce intense red spots. It is designed for ELISpot assays using PVDF membranes and is also compatible with chromogenic reactions in immunohistochemistry and western blot applications. This reagent is made for ELISpot applications.
Applications
ELISpot
Sample Type
Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.
Components
AEC Dilution 10mL, 1 bottle; AEC Solution I (20×) 500µl, 1 vial; AEC Solution II (20×) 500µl, 1 vial; AEC Solution III (200×) 50µl, 1 vial.
Storage
Shipped at 4°C. The kit should be stored at 2-8°C. Shelf life is 12 months from the date of receipt.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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