Assay Buffer (A326100)

The Assay buffer should be used for dilution of samples, standard and detection antibody. All samples should be diluted at least 2x in Assay buffer. Do not use undiluted serum/plasma samples in the assay. Sample dilution can be made either in separate tubes or directly in the plate. If sample dilution is made directly in the plate, Assay buffer should be added prior to addition of the sample. Important! Do not use the Assay buffer for dilution of Streptavidin conjugates. We recommend the use of an incubation buffer e.g. PBS with 0.05% Tween 20 containing 0.1% BSA for dilution of conjugates.