Coumarin 343 is a blue emitting fluorophore with an emission maximum around 480 nm. This dye forms a FRET pair with fluorescein, and can harvest blue light energy for the subsequent transfer to other fluorophores. The azide derivative can be conjugated with alkynes in a copper-catalyzed and copper-free Click chemistry reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye, and the azide function.
Absorption Maxima
437 nm
Extinction Coefficient
39000 M-1cm-1
Emission Maxima
477 nm
Fluorescence Quantum Yield
0.63
CF260
0.29
CF280
0.24
Mass Spec M+ Shift after Conjugation
480.3
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C25H32N6O4
Molecular Weight
480.56 kDa
Product Form
Yellow solid.
Solubility
Good in DMF and DMSO.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
