Coumarin 343 X NHS ester (A270132)

Coumarin 343 X NHS ester

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester. This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled. This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.

437 nm

39000 M^-1cm^-1

477 nm

0.63

946123-12-2

0.24

95% (by ¹H NMR and HPLC-MS).

C₂₆H₂₉N₃O₇

495.52 kDa

Yellow solid.

Good in DCM, DMSO, and DMF.

Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.

This product is for research use only. It is not intended for diagnostic or therapeutic use.