Cyanine 5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide. This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties. Cyanine 5 hydrazide replaces carbonyl-reactive Cy5® and DyLight 649 dyes.
Absorption Maxima
646 nm
Extinction Coefficient
250000 M-1cm-1
Emission Maxima
662 nm
Fluorescence Quantum Yield
0.2
CAS Number
1427705-31-4
CF260
0.03
CF280
0.04
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C32H42Cl2N4O
Molecular Weight
569.61 kDa
Product Form
Dark blue powder.
Solubility
Moderate solubility in water and good in polar organic solvents (DMF, DMSO, alcohols).
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
