Cyanine 5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines. This reactive derivative is useful for the synthesis of qPCR probes having Cyanine 5 at 5'-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent. Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.
Absorption Maxima
646 nm
Extinction Coefficient
250000 M-1cm-1
Emission Maxima
662 nm
Fluorescence Quantum Yield
0.2
CF260
0.03
CF280
0.04
Purity
85% (by 1H and 31P NMR, and HPLC-MS).
Molecular Formula
C47H67N5ClO3P
Molecular Weight
816.49 kDa
Product Form
Dark colored solid.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
