DiO is a green fluorescent lipophilic dye used to label cell membranes for cell tracking and fluorescence microscopy studies.
Applications
Fluorescence Microscopy, Flow Cytometry
Principle of Assay
DiO is a green fluorescent lipophilic tracer that becomes strongly fluorescent upon insertion into membranes, making it useful for plasma membrane labeling.
Sample Type
Live or fixed cells
Assay Time
20-40 minutes
Absorption Maxima
484 nm (MeOH)
Emission Maxima
501 nm (MeOH)
CAS Number
34215-57-1
Molecular Formula
C53H85ClN2O6
Molecular Weight
882
Product Form
Yellow solid.
Solubility
Soluble in Ethanol, anhydrous DMSO, and anhydrous DMF. Solubility of approximately 10 mg/ml in anhydrous DMSO. When it is difficult to dissolve, it can be properly heated or ultrasonically treated.
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
