Principle of Assay
Elite PAP pen provides a thin, film-like water repellent barrier when a circle is drawn around the specimen on the glass slide. This barrier creates the proper surface tension to hold antibody solution within the target area on the slide. This precludes the need for wiping around the specimen during the staining procedure. Since the reagent is guarded by the hydrophobic circle around the tissue, it minimizes the undesirable dilution of reagents with the wash buffer. This pen can be used for PAP, ABC, immunofluorescence, frozen sections and in situ hybridization procedures.
