Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR. Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition. HEX exhibits an excitation peak at 535 nm and an emission peak at 555 in green-yellow area, and it can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C24H12N4Cl6O6
Molecular Weight
665.09 kDa
Product Form
Orange solid.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.