Hoechst 33258 is a membrane-permeable blue fluorescent dye that binds the minor groove of DNA and is widely used for nuclear staining, apoptosis analysis, and cell cycle studies.
Sample Type
Formaldehyde-fixed and permeabilized tissue sections and cultured cells
Assay Time
10-20 minutes
Absorption Maxima
352 nm (with DNA), 346 nm (without DNA)
Emission Maxima
461 nm (with DNA), 460 nm (without DNA)
CAS Number
23491-45-4
Molecular Formula
C25H24N6O 3(HCl)
Molecular Weight
533.88
Solubility
Soluble in water.
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
