Hoechst 33342 is a cell-permeable blue fluorescent DNA stain that binds the minor groove of DNA and is commonly used as a nuclear counterstain and in cell cycle or apoptosis assays.
Sample Type
Live or fixed cultured cells
Assay Time
10-20 minutes
Absorption Maxima
350 nm (with DNA), 346 nm (without DNA)
Emission Maxima
461 nm (with DNA), 460 nm (without DNA)
CAS Number
23491-52-3
Molecular Formula
C27H28N6O 3(HCl) 3(H2O)
Molecular Weight
615.99
Product Form
Yellow solid.
Solubility
Soluble in water.
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
