Human eIF2A Knockdown Validation Kit (A349608)

Human eIF2A Knockdown Validation Kit

The shRNA-mediated knockdown cell lysate kit for eIF2A in HepG2 cells has been validated by Western blot analysis. The kit includes eIF2A knockdown cell lysate, matched wild-type cell lysate, and a knockdown-validated anti-eIF2A monoclonal antibody for confirmation of target specificity in Western blot applications.

WB

HepG2

Cell Lysate: 2 mg/mL, Antibody: lot specific

Liquid

Shipped at 4°C. Upon delivery store the antibody at -20°C and cell lysate at -80°C for up to 1 year.

Instructions for Use: This knockdown cell lysate is designed to be used alongside wild-type HepG2 cell lysate. For Western blotting, we recommend running both wild-type and knockdown lysates on the same gel, ensuring that each well is loaded with equal volumes and equivalent amounts of total protein.