The shRNA-mediated knockdown cell lysate kit for HuR in HeLa cells has been validated by Western blot analysis. The kit includes HuR knockdown cell lysate, matched wild-type cell lysate, and a knockdown-validated anti-HuR monoclonal antibody for confirmation of target specificity in Western blot applications.
Applications
WB
Sample Type
HeLa
Components
1. WB-Validated HuR Knockdown Cell Lysate (100 µg) 2. WT Cell Lysate (100 µg) 3. Verification Tool: Knockdown-Validated Human HuR Knockdown Validation Kit (A349283) (5 µL)
Concentration
Cell Lysate: 2 mg/mL, Antibody: lot specific
Product Form
Liquid
Storage
Shipped at 4°C. Upon delivery store the antibody at -20°C and cell lysate at -80°C for up to 1 year.
General Notes
Instructions for Use: This knockdown cell lysate is intended to be used together with wild-type HeLa cell lysate. For Western blotting, we recommend running both wild-type and knockdown lysates on the same gel, loading each well with equal volumes and equal amounts of total protein.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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