The shRNA-mediated knockdown cell lysate kit for PAI 1 in HT-1080 cells has been validated by Western blot analysis. The kit includes PAI 1 knockdown cell lysate, matched wild-type cell lysate, and a knockdown-validated anti-PAI 1 monoclonal antibody for confirmation of target specificity in Western blot applications.
Applications
WB
Sample Type
HT-1080
Components
1. WB-Validated PAI 1 Knockdown Cell Lysate (100 µg) 2. WT Cell Lysate (100 µg) 3. Verification Tool: Knockdown-Validated Human PAI 1 Knockdown Validation Kit (A349938) (5 µL)
Concentration
Cell Lysate: 2 mg/mL, Antibody: lot specific
Product Form
Liquid
Storage
Shipped at 4°C. Upon delivery store the antibody at -20°C and cell lysate at -80°C for up to 1 year.
General Notes
Instructions for Use: This knockdown cell lysate is intended to be used together with wild-type HT-1080 cell lysate. For Western blotting, we recommend running both wild-type and knockdown lysates on the same gel, ensuring each well is loaded with equal volumes and equal amounts of total protein.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Human PAI 1 Knockdown Validation Kit (A349893)? Please let us know so that we can list the citation on this page.
