The shRNA-mediated knockdown cell lysate kit for RNase H in HepG2 cells has been validated by Western blot analysis. The kit includes RNase H knockdown cell lysate, matched wild-type cell lysate, and a knockdown-validated anti-RNase H monoclonal antibody for confirmation of target specificity in Western blot applications.
Applications
WB
Sample Type
HepG2
Components
1. WB-Validated RNase H Knockdown Cell Lysate (100 µg) 2. WT Cell Lysate (100 µg) 3. Verification Tool: Knockdown-Validated Human RNase H Knockdown Validation Kit (A350162) (5 µL)
Concentration
Cell Lysate: 2 mg/mL, Antibody: lot specific
Product Form
Liquid
Storage
Shipped at 4°C. Upon delivery store the antibody at -20°C and cell lysate at -80°C for up to 1 year.
General Notes
Instructions for Use: This knockdown cell lysate is designed to be used alongside wild-type HepG2 cell lysate. For Western blotting, we recommend running both wild-type and knockdown lysates on the same gel, ensuring that each well is loaded with equal volumes and equivalent amounts of total protein.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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