Human UBE4A Knockdown Validation Kit (A349836)

Human UBE4A Knockdown Validation Kit

The shRNA-mediated knockdown cell lysate kit for UBE4A in HeLa cells has been validated by Western blot analysis. The kit includes UBE4A knockdown cell lysate, matched wild-type cell lysate, and a knockdown-validated anti-UBE4A monoclonal antibody for confirmation of target specificity in Western blot applications.

WB

HeLa

Cell Lysate: 2 mg/mL, Antibody: lot specific

Liquid

Shipped at 4°C. Upon delivery store the antibody at -20°C and cell lysate at -80°C for up to 1 year.

Instructions for Use: This knockdown cell lysate is intended to be used together with wild-type HeLa cell lysate. For Western blotting, we recommend running both wild-type and knockdown lysates on the same gel, loading each well with equal volumes and equal amounts of total protein.