Principle of Assay
Mouse Alkaline Phosphatase (AP) Detection Reagent is a non-biotin one-step detection reagent suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The Mouse Alkaline Phosphatase (AP) Detection Reagent may also be used with blood smears, cytosmears, and cell preparations. Mouse Alkaline Phosphatase (AP) Detection Reagent has been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step Mouse Alkaline Phosphatase (AP) Detection Reagent enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. Mouse Alkaline Phosphatase (AP) Detection Reagent is suitable for use with mouse all mouse antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays.
