Principle of Assay
The Mouse/Rabbit Alkaline Phosphatase (AP) Detection Reagent is suitable for use with all mouse and rabbit antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays. Mouse/Rabbit Alkaline Phosphatase (AP) Detection Reagent is a non-biotin one-step detection reagent suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The Mouse/Rabbit Alkaline Phosphatase (AP) Detection Reagent may also be used with blood smears, cytosmears, and cell preparations. The Mouse/Rabbit Alkaline Phosphatase (AP) Detection Reagent has been developed by directly labeling anti-rabbit and anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse and rabbit antibodies with a single reagent. Nuclear, cytoplasmic and membrane antigens in different types of tissues can be detected readily. The single step Mouse/Rabbit Alkaline Phosphatase (AP) Detection Reagent enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.
