Add the substrate after incubation with alkaline phosphatase and subsequent washing. Incubate for typically 30-90 minutes at room temperature. The substrate produces a yellow product. Read the absorbance at 405-410 nm.
Components
p-Nitrophenyl Phosphate and Diethanolamine Buffer.
CAS Number
333338-18-4
Molecular Formula
O2NC6H4OP(O)(ONa)2 · 6H2O
Molecular Weight
371.14 Da
Product Form
Liquid
Storage
Shipped at +4°C. Store at +4°C.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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