Principle of Assay
10X Citrate Buffer For Heat Induced Epitope Recovery, pH 6.0 is designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. The use of this buffer in combination with heat (often by microwave, water bath, or pressure cooker) has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue. This buffer is supplied as a 10X stock solution. The Antigen Retrieval technique is a novel method for the recovery of antigens from formalin-fixed, paraffin-embedded tissue. It consists of heating tissue sections in the presence of an antigen retrieval solution. The quality of the staining result is largely dependent on strict adherence to the antigen retrieval protocol. If the antigens are incompletely retrieved, the staining is light and the background may be high. Performs four steps simultaneously: deparaffinization, rehydration, antigen retrieval, and blocks endogenous peroxidase and alkaline phosphatase. Eliminates the use of alcohols and xylene in deparaffinization process. Saves money and hazardous disposal. When used in conjunction with mounting medium for mounting slides, the entire process from beginning to end is alcohol and xylene-free, thereby eliminating the use of hazardous chemicals.
